體外血栓模型設計及標準化探討_《華西醫學》

作者：

喻琴梅 ¹ , 周翔 ² ,  牛挺 ¹

四川大學華西醫院（成都，610041）1血液科及血液學研究室，2超聲診斷科;

關鍵詞：

溶栓體外血栓血栓模型標準化

DOI：

CNKI: 51-1356/R.20120214.1340.010

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針對栓塞血管及時、快速溶栓是治療心腦、肢體等血管血栓性疾病的關鍵之一。但溶栓過程中的出血和再栓塞等問題始終未得到很好的解決，仍需要大量的體外溶栓實驗研究。體外血栓模型的設計和完善是每個體外實驗研究的必需技術，且直接影響到溶栓效果的評價，但目前國內外尚缺乏統一、標準的模型設計。現就近年來文獻報道的常用體外血栓形成模型及其標準化進行探討。

引用本文： 喻琴梅,周翔,牛挺. 體外血栓模型設計及標準化探討. 華西醫學, 2012, 27(2): 210-212. doi: CNKI: 51-1356/R.20120214.1340.010 復制

1.	Holland CK, Vaidya SS, Datta S, et al. Ultrasound-enhanced tissue plasminogen activator thrombolysis in an invitro porcine clot model[J]. Thromb Res, 2008, 121(5): 663-673.
2.	Shaw GJ, Bavani N, Dhamija A, et al. Effect of mild hypothermia on the thrombolytic efficacy of 120 kHz ultrasound enhanced thrombolysis in an in-vitro human clot model[J]. Thromb Res, 2006, 117(5): 603-608.
3.	Meretoja A, Tatlisumak T. Thrombolytic therapy in acute ischemic stroke-basic concepts[J]. Curr Vasc Pharmacol, 2006, 4(1): 31-44.
4.	Esmon CT .Basic mechanisms and pathogenesis of venous thrombosis[J]. Blood Rev, 2009, 23(5): 225-229.
5.	Krueger K, Deissler P, Coburger S, et al. How thrombus model impacts the in vitro study of interventional thrombectomy procedures[J]. Invest Radiol, 2004, 39(10): 641-648.
6.	楊建軍, 宋雪丹, 陳海英, 等. 家犬自體血栓性腦梗死模型的建立[J]. 東南國防醫藥, 2009, 11(3): 196-198.
7.	嚴碧歌. 血栓形成過程與其密度的相關性研究[J]. 西北大學學報·自然科學版, 2006, 36(3): 356-358.
8.	Kirchhof K, Welzel T, Zoubaa S, et al. New method of embolus preparation for standardized embolic stroke in rabbits[J]. Stroke, 2002, 33(9): 2329-2333.
9.	Grimm J, Jahnke T, Muhle C, et al. Influence of thrombus age on the mechanical thrombectomy efficacy of the amplatz thrombectomy device in vitro[J]. Cardiovasc Intervent Radiol, 2003, 26(3): 265-268.
10.	Grimm J, Müller-Hülsbeck S, Heller M. Comparison of the mechanical thrombectomy efficacy of the amplatz thrombectomy device and the cragg thrombolytic brush in vitro[J]. Invest Radiol, 2001, 36(4): 204-209.
11.	華興, 高云華, 劉政, 等. 輻照時間與血栓凝齡對超聲波體外溶栓的影響[J]. 臨床超聲醫學雜志, 2007, 9(4): 193-195.
12.	杜寶琮, 杜寶民, 翟桂琴. 體外治療量超聲對血栓溶解效應的影響[J]. 中華物理醫學與康雜志, 2002, 24(7): 417-420.
13.	徐亞麗, 高云華, 劉政, 等. 超聲聯合微泡溶解體外血栓聲像圖改變與病理對照研究[J]. 中國超聲醫學雜志, 2006, 22(3): 164-167.
14.	Terres W, Beythien C, Kupper W, et al. Effects of aspirin and prostaglandin E1 on in vitro thrombolysis with urokinase. Evidence for a possible role of inhibiting platelet activity in thrombolysis[J]. Circulation, 1989, 79(6): 1309-1314.
15.	Torno MD, Kaminski MD, Xie Y, et al. Improvement of in vitro thrombolysis employing magnetically-guided microspheres[J]. Thromb Res, 2008, 121(6): 799-811.
16.	Pfaffenberger S, Devcic-Kuhar B, Kollmann C, et al. Can a commercial diagnostic ultrasound device accelerate thrombolysis? An in vitro skull model[J]. Stroke, 2005, 36(1): 124-128.
17.	黨京丹. 血栓與止血試驗診斷的研究新進展[J]. 當代醫學, 2009, 15(22): 13-14.
18.	Müller-Hülsbeck S, Grimm J, Suwelack D, et al. Mechanical assistance for enhancement of in vitro effectiveness for hydryodynamic thrombectomy [J]. Invest Radiol, 1999, 34(11): 699-677.

1. Holland CK, Vaidya SS, Datta S, et al. Ultrasound-enhanced tissue plasminogen activator thrombolysis in an invitro porcine clot model[J]. Thromb Res, 2008, 121(5): 663-673.
2. Shaw GJ, Bavani N, Dhamija A, et al. Effect of mild hypothermia on the thrombolytic efficacy of 120 kHz ultrasound enhanced thrombolysis in an in-vitro human clot model[J]. Thromb Res, 2006, 117(5): 603-608.
3. Meretoja A, Tatlisumak T. Thrombolytic therapy in acute ischemic stroke-basic concepts[J]. Curr Vasc Pharmacol, 2006, 4(1): 31-44.
4. Esmon CT .Basic mechanisms and pathogenesis of venous thrombosis[J]. Blood Rev, 2009, 23(5): 225-229.
5. Krueger K, Deissler P, Coburger S, et al. How thrombus model impacts the in vitro study of interventional thrombectomy procedures[J]. Invest Radiol, 2004, 39(10): 641-648.
6. 楊建軍, 宋雪丹, 陳海英, 等. 家犬自體血栓性腦梗死模型的建立[J]. 東南國防醫藥, 2009, 11(3): 196-198.
7. 嚴碧歌. 血栓形成過程與其密度的相關性研究[J]. 西北大學學報·自然科學版, 2006, 36(3): 356-358.
8. Kirchhof K, Welzel T, Zoubaa S, et al. New method of embolus preparation for standardized embolic stroke in rabbits[J]. Stroke, 2002, 33(9): 2329-2333.
9. Grimm J, Jahnke T, Muhle C, et al. Influence of thrombus age on the mechanical thrombectomy efficacy of the amplatz thrombectomy device in vitro[J]. Cardiovasc Intervent Radiol, 2003, 26(3): 265-268.
10. Grimm J, Müller-Hülsbeck S, Heller M. Comparison of the mechanical thrombectomy efficacy of the amplatz thrombectomy device and the cragg thrombolytic brush in vitro[J]. Invest Radiol, 2001, 36(4): 204-209.
11. 華興, 高云華, 劉政, 等. 輻照時間與血栓凝齡對超聲波體外溶栓的影響[J]. 臨床超聲醫學雜志, 2007, 9(4): 193-195.
12. 杜寶琮, 杜寶民, 翟桂琴. 體外治療量超聲對血栓溶解效應的影響[J]. 中華物理醫學與康雜志, 2002, 24(7): 417-420.
13. 徐亞麗, 高云華, 劉政, 等. 超聲聯合微泡溶解體外血栓聲像圖改變與病理對照研究[J]. 中國超聲醫學雜志, 2006, 22(3): 164-167.
14. Terres W, Beythien C, Kupper W, et al. Effects of aspirin and prostaglandin E1 on in vitro thrombolysis with urokinase. Evidence for a possible role of inhibiting platelet activity in thrombolysis[J]. Circulation, 1989, 79(6): 1309-1314.
15. Torno MD, Kaminski MD, Xie Y, et al. Improvement of in vitro thrombolysis employing magnetically-guided microspheres[J]. Thromb Res, 2008, 121(6): 799-811.
16. Pfaffenberger S, Devcic-Kuhar B, Kollmann C, et al. Can a commercial diagnostic ultrasound device accelerate thrombolysis? An in vitro skull model[J]. Stroke, 2005, 36(1): 124-128.
17. 黨京丹. 血栓與止血試驗診斷的研究新進展[J]. 當代醫學, 2009, 15(22): 13-14.
18. Müller-Hülsbeck S, Grimm J, Suwelack D, et al. Mechanical assistance for enhancement of in vitro effectiveness for hydryodynamic thrombectomy [J]. Invest Radiol, 1999, 34(11): 699-677.

《華西醫學》

體外血栓模型設計及標準化探討

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《華西醫學》

體外血栓模型設計及標準化探討

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